Enquiry

WIDAL SLIDE

 Place one drop of positive control on one reaction circles of the slide.
Pipette one drop of Isotonic saline on the next reaction cirlcle. (Negative Control).
Pipette one drop of the patient serum to be tested onto the remaining four reaction circles.
Add one drop of Widal TEST antigen suspension ‘H’ to the first two reaction circles. (PC & NC).
Add one drop each of ‘O’, ‘H’, ‘AH’ and ‘BH’ antigens to the remaining four reaction circles.
Mix contents of each circle uniformly over the entire circle with separate mixing sticks.
Rock the slide, gen